Figure 1.

Changes in mean ascorbic acid concentration in human milk. Triplicate samples of human milk, standardized to 42 mg/L of ascorbic acid at baseline, were collected. The samples were analyzed for ascorbic acid using normal-phase high performance liquid chromatography and compared to a standard curve. The mean concentrations for each triplicate sample were calculated at baseline, 5, 10, 15, and 20 minutes. Results represent the mean values (± SD) of three experiments displayed as a percent of the baseline level.